The molecular mechanism of retinoblastoma (RB) is complex, involving the abnormal regulation of many genes and signaling pathways. The objective of this research was to explore the molecular mechanisms underlying the role of Circ_0082415 in the progression of retinoblastoma (RB), with particular emphasis on its suppressive impact on the translation of MKLN1 and its consequent influence on the advancement of RB. The study quantified the expression levels of Circ_0082415 in both RB cells and normal retinal cells using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and subsequently analyzed the trend of Circ_0082415 expression throughout the progression of RB. The interaction mechanism between Circ_0082415 and MKLN1 mRNA was investigated by bioinformatics analysis and double luciferase reporter gene experiment. The expression of Circ_0082415 was markedly reduced in retinoblastoma (RB) cells, and its expression level exhibited a negative correlation with RB progression. Notably, the upregulation of Circ_0082415 significantly suppressed the proliferation, migration, and invasion of RB cells. A dual luciferase reporter gene assay substantiated the interaction between Circ_0082415 and MKLN1 mRNA, elucidating the mechanism through which Circ_0082415 exerts its anti-tumor effects by impeding the translation of MKLN1.